fig3

Figure 3. Pediococcus acidilactici CCFM6432’s effect on the gut microbiota of depressed patients. (A) α-diversity - Chao1 index; ^**P < 0.01 in paired t-test; (B-D) PCA of gut microbiota composition; PERMANOVA was used to assess β-diversity differences between groups; (E) LEfSe analysis of differential microbial species between groups (Wilcoxon rank-sum test, α 2.0 were used as the threshold); (F and G) Predicted genomic functions of gut microbiota and enrichment analysis of immune and neural function-related signaling pathways. The metagenomic composition was predicted from 16S rRNA sequences and annotated based on the reference database (KEGG). ^#P < 0.05 in the unpaired t-tests. PCA: Principal component analysis; PERMANOVA: permutational multivariate analysis of variance; LEfSe: linear discriminant analysis effect size; LDA: linear discriminant analysis; KEGG: Kyoto Encyclopedia of Genes and Genomes.