fig1

Figure 1. Experimental Overview and evaluation of bioinformatics workflow. (A) Flowchart depicting mouse fecal sample processing workflow and MS data acquisition. (B) Flowchart depicting different bioinformatic workflows tested in this study. Histograms of the total number of peptides and proteins identified with the different bioinformatics processing workflows as shown in (B) for (C) DDA and (D) DIA datasets, respectively. MS: Mass spectrometry; DDA: data-dependent acquisition; DIA: data-independent acquisition.