fig3

Figure 3. Microbiota profiles in recipient mice colonized with different porcine stool samples (CTRL or RL donors; blue and red, respectively) and fed either CD or CA diet (light and dark colors, respectively). (A) Generalized Unifrac distances shown as NMDS plot (PERMANOVA P.adj = 0.057); (B) Richness and Shannon effective counts for each donor-diet combination; (C) Comparative analysis of dominant SOTUs (mean relative abundance > 1% in at least one group). The columns, each representing a donor-diet combination, were clustered by Euclidean distance. The prevalence of the given SOTUs in the donor inoculum is indicated by bars on the right side. The closest taxonomic hit (based on EZBiocloud) of each SOTU is indicated by species name and percentage of sequence identity in parentheses; (D) Prevalent and abundant SOTUs (present in > 80% of all recipient mice; rel. abund. > 1% in at least one group) highlighting differences between the groups; (E) Major differences in the occurrence of dominant bacterial families. The criteria for selection/testing were the same as in (D). Statistics: Kruskal Wallis with Dunn’s multiple comparison, Benjamini-Hochberg correction (*P.adj < 0.05; **P.adj <0.01; ***P.adj < 0.001; ****P.adj < 0.0001). A previous version of this figure was published in the PhD thesis of Esther Wortmann (first author)^[40]. CTRL: Control donor microbiota, i.e., mice were colonized with feces from pigs fed the CTRL diet; RL: RL donor microbiota, i.e., mice were colonized with feces from pigs fed the RL diet; CD: control diet (recipient mice); CA: cholic acid-supplemented diet (recipient mice); NMDS: non-metric multidimensional scaling.