fig2

Figure 2. Fecal microbiota analysis using 16S rRNA gene amplicon sequencing to assess transfer efficiency. (A) Richness and Shannon effective number of species. Donor microbiota types are indicated by colors (CTRL, blue; RL, red). The different donors for each microbiota are indicated by symbols; (B) Microbiota profiles shown as NMDS, based on generalized UniFrac distances; (C) Distribution of SOTUs (Venn diagram) detected in at least one donor pig or one recipient mouse. The total number of SOTUs was 269; (D) SOTUs detected in either all groups or only one microbiota type (donors or recipients), or that matched a reference 16S rRNA gene sequence of known DCA producers. Only positive values were plotted; (E) Heatmap of SOTUs, which were characteristic of each group (prevalent in 100% of the samples per group). The columns represent the donors and corresponding recipient mice. The color in boxes indicated relative abundances as mean values. Number of sequenced samples: left, donors (dark colors), n = 2 cryostocks per pig; right, recipients (pale colors), n = 10-13 mice for CTRL microbiota, light blue, n = 7-13 mice for RL microbiota, light red. A previous version of this figure was published in the PhD thesis of Esther Wortmann (first author)^[40]. CTRL: Control donor microbiota, i.e., mice were colonized with feces from pigs fed the CTRL diet; RL: RL donor microbiota, i.e., mice were colonized with feces from pigs fed the RL diet; NMDS: non-metric multidimensional scaling; SOTUs: specific molecular species; DCA: deoxycholic acid.