fig1

Figure 1. Phenotype of Apc^1638N/+ mice after FMT. (A) Number of intestinal lesions for each donor-diet combination. Bottom: example macroscopic images of intestines with confirmed tumors. Left: multiple tumors (adenoma) in the PAR of a germfree mouse on the CA diet (red circle), with two additional intestinal lesions in the duodenum (red arrowheads). Right: A tumor (adenoma) in the ileum of a CTRL mouse on the CA diet (red arrowhead). Exemplary histological analyses of two lesions are shown in Supplementary Figure 5; (B) Number of lesions per gut region; (C) Length of the small intestine and colon (mean ± standard deviation). A previous version of this figure was published in the PhD thesis of Esther Wortmann (first author)^[40]. In panels (A) and (B), the mean number of lesions is indicated by white lines. Statistics: Kruskal-Wallis followed by Dunn’s multiple comparisons with Benjamini-Hochberg adjustment (*P.adj < 0.05; **P.adj < 0.01; ***P.adj < 0.001; ****P.adj < 0.0001). FMT: Fecal microbiota transfer; PAR: periampullary region; CTRL: control donor microbiota, i.e., mice were colonized with feces from pigs fed the CTRL diet; CA: cholic acid-supplemented diet (recipient mice); GF: germfree; RL: RL donor microbiota, i.e., mice were colonized with feces from pigs fed the RL diet; CD: control diet (recipient mice).