fig6

Figure 6. SCFA modulation by cranberry extract supplementation and correlations with bacterial genera. (A) Mean ratio and (B) mean concentration ±SD over time of the three major SCFA for the six donors in the ascending and transverse colon during the control week (blue line) and the two subsequent weeks of treatment (week 1: light red, week 2: dark red). Statistically significant differences between control and treatment period are denoted for P < 0.05 (^*), P < 0.01 (^**), and P < 0.001 (^***) as determined by Kruskal-Wallis followed by post-hoc Dunn’s test. (C-F) Spearman correlations between the three major SCFA and bacterial genera in the transverse colon. Correlations were performed in the lumen (C and E) and mucus (D and F) environments separately during the first (C and D) and second week (E and F) of supplementation. Only negative and positive significant correlations (P < 0.05 Benjamini-Hochberg adjusted) that are greater than a -0.7 or 0.7 coefficient are shown, denoted in shades of orange and purple, respectively. SCFA: Short-chain fatty acid.