fig3

Figure 3. Bifidobacterium species shifts induced by the supplementation with a cranberry extract in the ascending colon. (A and B) Bifidobacterium genus behavior following the supplementation in the lumen (A) and mucus (B), respectively, as evaluated by Deseq2 analysis. The changes between the control and the second week of supplementation are significantly different at α = 0.05, as determined using a Wald Test with Benjamini-Hochberg multiple testing correction; (C and D) Volcano plots showing the ASVs assigned to Bifidobacterium species significantly affected by the supplementation as determined by the Wald test with Benjamini-Hochberg multiple testing correction in the lumen (C) and mucus (D) of the ascending colon. Deseq2 analysis of log2 fold-changes displays on the left of the x-axis (in blue) the ASVs depleted after the two weeks of supplementation, while on the right (in red), the ones enriched after the two weeks of supplementation. Each ASV sequence was assigned to the corresponding species based on RDP classifier. The y-axis shows the log-transformed adjusted P-value with the dashed line indicating the α = 0.05 significance threshold.