fig3
Figure 3. Metabolomics revealed specific metabolite changes attributed to the host, continuous flow, and F. prausnitzii A2-165. (A) The volcano plot of GuMI-NB vs. Static. P 2. Blue is significantly higher in static condition (Static) culture; red is significantly higher in GuMI-NB; (B) Metabolites mapped to glycolysis and Krebs cycle pathways. Metabolites highlighted in red, blue, and black are significantly higher, significantly lower, and not significantly changed in GuMI-NB vs. Static, respectively. Significance threshold: P 2. Metabolites in grey are not detected; (C) The volcano plot of metabolite changes in GuMI-FP vs. GuMI-NB. P 1.5. The rationale for lowering the threshold is that the continuous flow dilutes the produced metabolites. Therefore, a 50% change in the chemical concentration is considered significant; One-way ANOVA was performed for each compound in (B-D) to compare the differences between Static, GuMI-NB, and GuMI-FP. Note that the peak area ratio can not be used to compare the levels of different compounds; (E) Nucleoside hydrolase gene in the genome of F. prausnitzii A2-165. Gene ID: CG447_RS10420; accession ID of its predicted protein: C7H870 (UniProt). GuMI-NB: GuMI without bacteria; GuMI-FP: GuMI with
