fig2

Figure 2. Metabolite profile in the apical compartment in static condition (Static) and GuMI culture with and without bacteria. (A) The principal component analysis plot indicates a clear separation of the analyzed metabolome among the three groups. In total, 76 detected compounds were included; (B) A distance plot of all the samples shows the inter-batch variation. In total, four batches of GuMI experiments were carried out. The samples are from four experiments as follows: experiment 1: Static_1 and 2, GuMI-NB_1, GuMI-FP_1 and 2; experiment 2: Static_3, 4, and 5, GuMI-NB_2 and 3, GuMI-FP_3 and 4; experiment 3: Static_6, GuMI-NB_4 and 5, GuMI-FP_5 and 6; experiment 4: Static_7 and 8, GuMI-NB_6, 7, and 8, GuMI-FP_7 and 8; (C) Heatmap of detected metabolites in Static, GuMI-NB, and GuMI-FP. Clusters 1, 2, 3, and 4 indicate the metabolites accumulated in specific conditions. GuMI-NB: GuMI without bacteria; GuMI-FP: GuMI with F. prausnitzii.