fig1

Figure 1. Growth and metabolic activity of probiotic strains in single and co-culture conditions in the presence of prebiotics or intact/hydrolyzed protein. (A) Growth of Bb (top) and Lf (bottom) in co-culture (1:1 ratio), in the presence of iPF without prebiotics (green), with GOS (blue), or eHF with GOS (orange); (B) Formation of Acetate; (C) Propionate; (D) Butyrate; or (E) Lactate or (F) volume of gas formation in ml after 24 and/or 48 h after growth of Bb as single culture (open bars), Lf as single culture (dashed lines bar) or both strains as co-culture (filled bars) in the different formulae. Error shown is the standard deviation. n = 3 per time point. Selection of significant differences are marked with a ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. iPF: Intact protein formula; GOS: galactooligosaccharide, eHF: extensively hydrolyzed formula; Bb: B. breve DSM 32583; Lf: L. fermentum CECT 5716.