fig2

Figure 2. Akkermansia species are distinguishable by their estimated metabolic capabilities and fatty acid composition. (A) Unique core gene clusters were defined as those present in all genomes of one species, but not present in every genome of another species. Shared core gene clusters (n = 1,192) were defined as those present in all genomes of multiple species. All core gene clusters were categorized by these definitions and tallied to define the sizes of the A. muciniphila, A. massiliensis, and A. biwaensis core genomes; (B) Estimated metabolic enrichment analysis indicates the gain or loss of some metabolic pathways in Akkermansia phylogroups. Blue indicates pathway presence, calculated by pathway completeness of 50% or greater; (C) A principal components analysis was performed to compare fatty acid composition across species. The principal component scores distinguish three clusters comprised primarily of either A. muciniphila and AmV, A. massiliensis, or A. biwaensis and AmVI, as indicated by dotted ellipses. Isolates are colored by species; (D) Average fatty acid composition by C14:0 iso and C15:0 iso distinguishes A. massiliensis from other Akkermansia species.