fig3

Figure 3. Heatmap of relative abundances of ASVs significantly changed by Ch and the WD which can be assigned into three different groups based on the changes in relative abundance between the groups. Group 1 (black): 14 ASV abundances are significantly reversed back toward ND levels after the introduction of cholestyramine to a WD. Group 2 (purple): abundance of 31 ASVs is altered by cholestyramine alone. Group 3 (orange):17 ASVs are altered by a WD, regardless of treatment with cholestyramine. Rows (ASVs) of heatmaps were clustered first by abundance in experiment 1 and the same order was used for experiment 2. Significant ASVs were required to be consistent in fold-change direction across both experiments (Mann-Whitney U P-value < 0.05). ASVs: Amplicon sequence variants; Ch: cholestyramine; ND: normal diet; WD: Western diet.