fig1

Figure 1. Alpha diversity at ASV level. Comparison of (A) richness and (E) evenness between FAP-NOS and control groups; Visualization of the effect of (B) probiotics treatment in the last three months (FALSE vs. TRUE); (C) fecal dry weight; and (D) fecal pH on gut microbial richness. Boxplot with box elements showing upper quantile, lower quantile, and median. Whiskers extend from the upper/lower quantile to ± 1.5 iqr range or the highest/lowest value. Outliers are indicated as black points. Points in the scatterplot display values for individual microbiota. Regression line is based on multiple linear regression model, and 95% confidence interval is displayed. All significances were calculated using a multiple linear regression model [Supplementary Table 3]. *P < 0.05; ****P < 0.0001. FAP-NOS: Functional abdominal pain-not otherwise specified; ns: not significant; ASV: amplicon sequence variant; iqr: interquartile range.