fig6
Figure 6. Carbohydrate analysis of B. breve I1-B. longum M12 interaction. TLC plates display standards, negative controls, and carbohydrate intake at 0, 24, and 48 h of the assay. (A) B. breve I1 monoculture; (B) B. breve I1 co-culture; (C) B. longum M12 monoculture; and (D) B. longum M12 co-culture. B. breve I1 was cultured in the bottom well, and B. longum M12 was cultured in the Transwell upper insert. LNnT: Lacto-N-neotetraose; LNT: lacto-N-tetraose; TLC: thin-layer chromatography; 2’-FL: 2’-fucosyllactose;