fig1
Figure 1. Diagram illustrating the PFNA operon of B. longum subsp. longum GT15, and the FN3 protein and its fragments (∆FN3.1 protein, 2D FN3, CD FN3) used in our experiments aimed at testing the protein’s ability to bind the cytokine TNFα. The genes of the PFNA operon are pkb2 (serine-threonine protein kinase Pkb2, BLGT_RS02820, AIW43409.1), fn3 (fibronectin type III do-main-containing protein, BLGT_RS02815, AIW43408.1), aaa-atp (AAA-ATPase MoxR, BLGT_RS02810, AIW43407.1), duf58 (hypothetical protein with DUF58 domain, BLGT_RS02805, AIW43406.1), tgm (transglutaminase, BLGT_RS02800, AIW43405.1), prpC (protein phosphatase, BLGT_RS02795, AIW43404.1), hypothetical protein (BLGT_RS02790, AIW43403.1), fha (FHA do-main-containing protein, BLGT_RS02785, AIW43402.1). SP: signal peptide; TM: transmembrane.