fig2
![Saponin treatment for eukaryotic DNA depletion alters the microbial DNA profiles by reducing the abundance of Gram-negative bacteria in metagenomics analyses](https://image.oaes.cc/ff6c2248-85fb-4b2e-93cc-e118edb6a87f/mrr2002.fig.2.jpg)
Figure 2. Gram-negative and Gram-positive bacterial ratio in different biological samples before and after saponin-based depletion protocol. Panel (A) indicates the percentages of Gram-negative (pink) and Gram-positive bacteria (green) on the total amount of bacterial DNA profiled in each biological sample before (NT) and after 2.5% wt/vol saponin-based depletion (T); panel (B) represents the percentages of Gram-negative (pink) and Gram-positive bacteria (green) detected in a sputum sample treated with different saponin amounts.