fig3

Figure 3. Validation of 16S metagenome-DRIP method using infant fecal samples. (A and B) Net increase of bacterial taxa is correlated with the relative abundance of the target species Bifidobacterium (A) but not with the number of bacterial taxa (species richness) (B) in samples (Pearson’s correlation coefficient analysis). Subject IDs are indicated (see Table 2). (C) Bray-Curtis dissimilarity indices between the Control and DRIP groups. The values were calculated by omitting the target species (Bifidobacterium). (D) The relative abundances (%) of bacterial taxa detected in infant fecal samples analyzed by the conventional 16S metagenome (Control) and 16S metagenome-DRIP (DRIP) methods. Asterisks (*) indicate that the reads corresponding to the target species (Bifidobacterium) were omitted for drawing the bar charts. Different colors are given to different taxa. The target species (Bifidobacterium) is shown in dark blue with a red frame. DRIP: Deeper Resolution using an Inhibitory Primer.