fig2

Figure 2. Proof-of-concept experiment using a mock sample. (A) The sequence alignment of the V3-V4 region of the 16S rRNA genes of 14 bacterial species (B. breve JCM 1192, B. longum subsp. longum JCM 1217, B. pseudocatenulatum JCM 1200, C. aerofaciens JCM 10188, B. wexlerae JCM 17041, C. comes ATCC 27758, F. prausnitzii JCM 31915, L. gasseri JCM 1131, L. reuteri JCM 1112, B. fragilis JCM 11019, B. ovatus JCM 5824, B. uniformis JCM 5828, P. vulgatus JCM 5826, and E. coli MG1655). The inhibitory primer sequences, which correspond to the V4 region, are boxed in red and black. (B) A mixture of the 14 bacterial genomes was subjected to the conventional 16S metagenome (Control) and 16S metagenome-DRIP (DRIP) analyses. The relative abundances of 14 species are shown in different colors. DRIP: Deeper Resolution using an Inhibitory Primer.