fig1

Figure 1. Schematic representation of the 16S metagenome-DRIP analysis. Amplification of the 16S rRNA genes of non-target (left) and target (right) species in the presence of an inhibitory primer specific for the target species. The biotinylated inhibitory primer (green) anneals to an internal site of the amplification target region, which leads to an aberrant short amplicon formation of the 16S rRNA gene of the target species. The short amplicon with the biotin-tag is bound by streptavidin beads and thereby removed from the first PCR mixture. The subsequent procedure is identical to the conventional 16S metagenome analysis. NGS: Next-generation sequencing.