fig3

Figure 3. Osteogenesis-related gene and protein expression levels: (A-C) Gene expression of OCN, RUNX2, and VEGF in MC3T3-E1 cells following treatment with BG-conditioned culture media for 7 and 14 days, respectively (n = 3); (D) and (F) Representative images of BMP2 and VEGF protein expression in MC3T3-E1 cells on days 7 and 14; (E) and (G) Quantification of BMP2 and VEGF protein expression levels by ImageJ. Data are presented as the mean ± SD (n = 3). *P< 0.05, **P < 0.01, ***P< 0.005, ****P< 0.0001, compared with control group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.005, ^####P < 0.0001, compared with each experimental group. OCN: Osteogenic genes osteocalcin; BMP2: Bone morphogenetic protein 2; VEGF: Vascular endothelial growth factor; BG: Bioactive glass; RUNX2: Runt-related transcription factor 2.