fig2

Figure 2. Osteogenesis ability evaluation after culturing in glass-conditioned culture media for different time points. (A) ALP staining quantification of MC3T3-E1 at 14 days (n = 4); (B) Quantification of ALP activity at 14 days; (C) Alizarin Red S staining at 14 and 21 days (n = 3); (D) Quantification of mineralized nodule formation at 14 and 21 days. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001, compared with the control group. #p < 0.05, ##P < 0.01, ###P < 0.005, ####P < 0.0001, compared with each experimental group. The bar represents 250 µm, and the values were normalized by comparison with the control group. ALP: Alkaline phosphatase.