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Figure 2. Exploring MAGEA3 expression in mice CRC and human CRC samples. (A) The mRNA level of MAGEA3 in tumor and peri-tumor colon tissues from AOM/DSS-treated mice compared to colon tissues in untreated mice; (B) The mRNA level of MAGEA3 in different human CRC cell lines HCT116, Caco-2, and HT29, compared to normal colon epithelial cell line FHC; (C and D) Images and quantification of Western blot of MAGEA3 in tumor and peri-tumor colon tissues from AOM/DSS-treated mice compared to colon tissues from untreated mice; (E and F) Representative images and quantification of immunohistochemistry staining of MAGEA3 in tumor and peri-tumor tissues of human CRC (scale bar: 100 μm). ns: no significance, ^*P < 0.05, ^**P < 0.01, ^****P < 0.0001. CRC: colorectal cancer; AOM: azoxymethane; DSS: dextran sulfate sodium.