fig8

Figure 8. Melatonin alleviates oxidative stress response and ferroptosis in P301S mouse brains. (A and B) Immunofluorescence labeling and confocal microscopy analysis showing the expression of GPX4 (red) and p-Tau (Ser396) (green) in the cortex and hippocampus of the P301S mouse brains. DAPI was used to detect the nuclei (blue). Scale bar = 25 μm; (C-G) Western blotting analysis showing that the protein expression levels of SOD1, GPX4, Nrf2, and xCT were significantly increased in melatonin-treated mice. β-actin served as the internal control; (H-J) GSH, ROS, and MDA production levels in the brains of P301S mice, as detected by the DCFH-DA method. Values are represented as mean ± SEM (n = 8). ^*P < 0.05. GPX4: Glutathione peroxidase 4; DAPI: 4’,6-diamidino-2-phenylindole; SOD1: superoxide dismutase 1; Nrf2: nuclear factor erythroid 2-related factor 2; xCT: cystine-glutamate antiporter; GSH: gamma-glutamyl-cysteinyl-glycine; ROS: reactive oxygen species; MDA: malondialdehyde; DCFH-DA: 2’,7’-dichlorofluorescein diacetate; SEM: standard error of the mean.