fig6

Figure 6. Melatonin increases hepcidin expression in the brain of P301S mice. (A) Immunohistochemical staining results of hepcidin in the cortex and hippocampus of P301S mice. Scale bar = 50 μm; (B and C) Western blotting analysis and quantification of hepcidin with β-actin as an internal control; (D) The hepcidin mRNA levels in the brain of P301S mice were significantly upregulated after melatonin treatment with GAPDH as an internal control. The values are presented as mean ± SEM (n = 8). ^*P < 0.05. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SEM: standard error of the mean.