fig3

Figure 3. Melatonin relieves synaptic dysfunction and neuronal damage in P301S mice. (A) Nissl staining indicating the functional states of neurons in the hippocampal CA1 region of melatonin- and vehicle-treated P301S mice. Scale bar = 20 μm; (B) Immunohistochemical staining results of MAP2 in the hippocampal CA1 region of P301S mice. Scale bar = 50 μm; (C-F) Immunoblotting results showing the levels of MAP2, NeuN, PSD95, and SYP in the P301S mouse brains after melatonin treatment. GAPDH served as an internal control. Values are represented as mean ± SEM (n = 8). ^*P < 0.05. MAP2: Microtubule-associated protein 2; PSD95: postsynaptic density protein 95; SYP: synaptophysin; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; SEM: standard error of the mean.