fig2

Figure 2. Melatonin ameliorates Tau hyperphosphorylation in P301S mice via multiple pathways. (A-G) Western blotting results showing the levels of phosphorylated Tau at sites Ser202, Ser396, Ser404, Thr181, and Thr231 in the brain of melatonin/-vehicle treated P301S mice; (H-K) Immunoblotting analysis of calpain1, p-CDK5, CDK5, and P35/25 in the brains after melatonin treatment; (L-O) Immunoblotting analysis of p-GSK3β (Ser9), p-GSK3α/β (Tyr279/Tyr216), and GSK3β in the brains after melatonin treatment. β-actin served as the internal control. The values are presented as mean ± SEM (n = 8). ^*P < 0.05. CDK5: Cyclin-dependent kinase 5; GSK3β: glycogen synthase kinase 3β; SEM: standard error of the mean.