fig1

Figure 1. (A) Changes in LPx of Cyprinus carpio muscle expressed as nM MDA/mg protein exposed to two concentrations of GLI (50 and 1,000 ng/L) for 12, 24, 48, 72 and 96 h. Values are the means of three replicates ± SE. *Significant differences from control group; ^abcdeDifferent letters represent statistically significant differences between concentrations (P < 0.05) ANOVA, and Tukey (P < 0.05); (B) Changes in PCC of Cyprinus carpio muscle, expressed in mmol C=O/mg protein, upon exposure to two concentrations of GLI (50 and 1,000 ng/L) for 12, 24, 48, 72 and 96 h. Values are the means of three replicates ± SE. *Significant differences from control group; ^abcdeDifferent letters represent statistically significant differences between concentrations (P < 0.05), ANOVA, and Tukey (P < 0.05); (C) SDS-PAGE Profile of myofibrillar proteins of Cyprinus carpio muscle exposed to two concentrations of GLI (50 and 1,000 ng/L) for 12, 24, 48, 72, and 96 h. Bands according to Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards #1610375; (D) Warner-Bratzler share force in Cyprinus carpio muscle exposed to two different GLI concentrations (C1: 50 ng/L and C2: 1,000 ng/L) for 0, 24, 48, 72, and 96 h. Values are the means of three replicates ± SE. *Significant differences from control group, ANOVA, and Tukey (P < 0.05). C1: Concentration 1 (50 ng/L); C2: concentration 2 (1,000 ng/L); C1₀: concentration 1 (50 ng/L) zero time; C2₀: concentration 2 (1,000 ng/L); C1₂₄: concentration 1 (50 ng/L) 24 h; C2₂₄: concentration 2 (1,000 ng/L) 24 h; C1₄₈: concentration 1 (50 ng/L) 48 h; C2₄₈: concentration 2 (1,000 ng/L) 48 h; C1₇₂: concentration 1 (50 ng/L) 72 h; C2₇₂: concentration 2 (1,000 ng/L) 72 h; C1₉₆: concentration 1 (50 ng/L) 96 h; C2₉₆: concentration 2 (1,000 ng/L) 96 h; LPx: lipid peroxidation; M: protein marker; MDA: malondialdehyde; PCC: protein carbonyl content; TC: test control.