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![Wearable electrochemical sensors for real-time monitoring in diabetes mellitus and associated complications](https://image.oaes.cc/80a26ea4-faaf-4179-aa6e-f69fdc5ed292/ss4002.fig.2.jpg)
Figure 2. Response mechanisms of enzymatic electrochemical sensors and enzyme immobilization methods. (A) Schematic illustration of the first-generation enzymatic sensor utilizes the concept of electrocatalytic detection; (B) Schematic representation of the second-generation enzymatic sensor incorporates redox mediators; (C) The third-generation enzymatic sensor; (D) Schematic illustration for physical enzyme immobilization of soaking method, drying method, entrapment method, and ionic binding, respectively; (E) Diagram illustrating enzyme immobilization of chemical methods of covalent bonding and crosslinking, respectively.