fig7

Figure 7. (A) Western blotting assay of Snail expression in MCF-7 cell line after transfection with the wtSnail plasmid. Densitometry for Snail/α-tubulin ratio was carried out using ImageJ software (version 1.53m); (B) Reporter analysis of Snail transcriptional (trans-repressor) activity after wtSnail transfection. wtSnail-transfected MCF-7 cells were subjected to transfection with the luciferase reporter gene construct bearing Snail-sensitive wild-type E-cadherin promoter sequences. The data reflect the mean ± S.D. from three independent assays; ^*P < 0.05 - in comparison with cells treated with empty vector (t-test was applied); (C) Immunoblotting analysis of the MCF-7 cell protein samples after wtSnail transfection. Densitometry for the indicated proteins/α-tubulin ratio was carried out using ImageJ software (version 1.53m); (D) Reporter assay of the ERα transcriptional activity in Snail-transfected cells. The data are the mean ± S.D. from three independent tests; ^*P < 0.05 - compared to cells treated with empty vector (t-test was used to compare two sample means); (E) Reporter assay of Snail transcriptional (trans-repressor) activity in MCF-7, MCF-7/T, and MCF-7/Rap cell sublines. The data reflect the mean ± S.D. from three independent trials; ^#,*P < 0.05 - compared to luciferase activity in MCF-7 cells, which is taken as 100 relative units (t-test was used to compare two sample means).