fig6

Figure 6. (A) Immunoblotting analysis of the general signaling proteins in MCF-7 cells after NR6A1 knockdown; (B) Immunoblotting analysis of the estrogen receptor α signaling proteins in MCF-7 cells after NR6A1 knockdown. Densitometry for the tested proteins/α-tubulin ratio was performed using ImageJ software (version 1.53m); (C) Reporter assay of the ERα-responsive element after NR6A1 knockdown; (D) Reporter assay of the BRCA1 promoter after NR6A1 knockdown. The data are the mean ± S.D. from three independent tests; ^*P < 0.05 - in comparison with cells treated with empty vector (t-test).