fig2

Figure 2. (A) The cross-resistance of MCF-7, MCF-7/T, and MCF-7/Rap cell sublines to tamoxifen and rapamycin. The cells were processed with the mentioned derivatives within 72 h, and the cell survival was measured by the MTT assay. The data reflect the mean value ± S.D. from three independent experiments, ^*P < 0.05 - compared to respective control cells, ^#P < 0.05 - vs. MCF-7 cells treated with respective drug (t-test was applied); (B) Reporter assay of the transcriptional activity of ERα. The relative luciferase activity (ERE-Luc) was measured in arbitrary units as the relation of luciferase to β-galactosidase activity. The data are the mean ± S.D. from three independent trials, ^*P < 0.05 - in comparison with MCF-7 and MCF-7/Rap cells, ^#P < 0.05 - vs. MCF-7 and MCF-7/T cells.