fig1

Figure 1. MCF-7 cell line sensitivity to antiestrogen tamoxifen (A) and mTOR inhibitor rapamycin (B). MCF-7 cell line was exposed to the mentioned compounds within 72 h, and the cell survival was measured by the MTT assay. The results represent the mean value ± S.D. from three independent experiments. (C) Western blotting of the MCF-7 protein samples exposed to 5 μM tamoxifen or 0.5 μM rapamycin within 24 h and protein samples of the MCF-7 cells and MCF-7/T and MCF-7/Rap sublines. PR - progesterone receptor, p-S6K - phosphorylated ribosomal protein S6 kinase, S6K - ribosomal protein S6 kinase, ERα - estrogen receptor α, CDK6 - cyclin-dependent kinase 6. To monitor the transfer quality, samples were visualized by incubating the membranes in Ponceau S dye solution^[41,42]. The α-tubulin antibodies were used as loading control. Densitometry for the tested proteins/α-tubulin ratio was carried out using ImageJ software (version 1.53m). One of two replicates is shown.