fig2

Figure 2. Activity of GLS1 inhibitors in MM cells. (A) MM cell lines and PBMC of normal control samples were cultured with varying concentrations of CB-839. After 72 h, the relative cell counts were assessed; (B-D) U266 cells were treated with the indicated concentrations of CB-839 for 24 h. The total amounts of NADP+ or NADPH and α-KG were analyzed as described in the materials and methods section. Statistical significance is denoted as ^****P < 0.0001 compared to the control. ns: not significant. (E) U266 cells were treated with the indicated concentrations of CB-839 for 24 h. Intracellular ATP levels in U266 cells (4 × 10⁴) were quantified. Statistical significance is indicated as ^****P < 0.0001 compared to the control. ns: not significant. GLS: Glutaminase; MM: multiple myeloma; PBMC: peripheral blood mononuclear cell; NADP+: nicotinamide-adenine-dinucleotide phosphate; NADPH: dihydro-nicotinamide-adenine-dinucleotide phosphate; α-KG: α-ketoglutarate; ATP: adenosine triphosphate.