fig3

Figure 3. Comparison of metabolic pathway dependence of TGCT cell lines. TGCT cell lines NTERA-2 (A) and NTERA-2 Cis (B), 1411HP (C), 1777NRpmet (D), 2102EP (E), 2102EP Cis (F), and H12.1 (G), H12.1 ODM (H) were treated with combination of two inhibitors, 2-DG inhibitor to inhibit glycolysis and oligomycin to inhibit the OXPHOS metabolic pathway. Absorbance (450 nm) and luminescence values were normalized to untreated TGCT cells. To determine the metabolic pathway dependence of individual TGCT cell lines, we compared changes in ATP and lactate production after the application of inhibitors. Data are presented as mean relative ATP and lactate amounts from three technical and three biological replicates. Error bars represent SD. ^*P ≤ 0.05; ^**P ≤ 0.01; ^***P ≤ 0.001. TGCT: Testicular germ cell tumors; OXPHOS: oxidative phosphorylation.