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Figure 5. miR-92b-5p regulates RAD51 expression and PI3K-AKT-mTOR pathway via modulating PTEN subcellular localization. (A and B) The expression and relative expression of PTEN in total protein, cytoplasmic and nuclear proteins in K562 and K562/DOX cells transfected with miR-92b-5p mimics or inhibitors; (C) Immunofluorescence of PTEN in K562 and K562/DOX cells transfected with miR-92b-5p mimics or inhibitors; (D and E) The expression and relative expression of RAD51 in total protein and nuclear proteins in K562 and K562/DOX cells transfected with miR-92b-5p mimics or inhibitors; (F-H) The expression and relative expression of PI3K-AKT-mTOR pathway in total protein and cytoplasmic protein in K562 and K562/DOX cells transfected with miR-92b-5p mimics or inhibitors. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, n = 3.