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From: Exosomal miR-92b-5p regulates N4BP1 to enhance PTEN mono-ubiquitination in doxorubicin-resistant AML
Figure 4. Analysis of NEDD4 expression, PTEN ubiquitination, and protein interactions in K562 and K562/DOX cells. (A) The expression of NEDD4 protein in K562 and K562/DOX cells transfected with miR-92b-5p mimics or inhibitors; (B) The expression of mono-ubiquitinated PTEN protein in K562 and K562/DOX cells; (C) NEDD4 and N4BP1, NEDD4 and PTEN endogenous Co-IP in K562 cells and K562/DOX cells. *P < 0.05, ***P < 0.001, n = 3. N4BP1: NEDD4 binding protein 1; Co-IP: co-immunoprecipitation.
