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Figure 3. miR-92b-5p binds to and suppresses N4BP1 expression in doxorubicin-resistant leukemia cells. (A) Binding site of miR-92b-5p to N4BP1 mRNA 3’-UTR; (B) The relative Luciferase activity in 293T cells co-transfected with miR-92b-5p mimics or miR-NC and N4BP1-3’-UTR-WT or N4BP1-3’-UTR-MUT; (C) The expression of N4BP1 mRNA in K562 and K562/DOX cells; (D) The expression of N4BP1 mRNA in K562 and K562/DOX cells transfected with miR-92b-5p mimics or inhibitors; (E-G) The expression of N4BP1 protein in K562 and K562 /DOX cells transfected with miR-92b-5p mimics or inhibitors. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, n = 3. N4BP1: NEDD4 binding protein 1.