fig6

From: Isocucurbitacin B inhibits glioma growth through PI3K/AKT pathways and increases glioma sensitivity to TMZ by inhibiting hsa-mir-1286a

Figure 6. (A and B) Dye content of FITC-12-dUTP was detected by FCM (^****P < 0.0001 vs. control group); (C and D) The apoptosis rate was increased by FCM. Q3-1 represents normal cells, Q3-4 represents viable apoptotic cells, Q3-2 represents late apoptotic cells, and Q3-1 represents mechanical destruction of cells (^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 vs. control group); (E and F) Protein expression of BCL-2 was analyzed using WB. Data are represented as mean ± SD (^*P < 0.05, ^**P < 0.01 vs. control group); (G) Expression of BCL-2 RNA was detected using RT-qPCR (n = 3) (^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. control group). FCM: Flow cytometry; BCL-2: B-cell lymphoma-2; WB: western blot; RT-qPCR: reverse transcription-quantitative polymerase chain reaction.