fig2

Figure 2. (A) Representative findings of matrigel invasion (upper panels), formation of lamellipodia and invadopodia [middle six panels: nuclei were counterstained with DAPI (blue fluorescence), and sphere formation (lower panels) of BxPC-3 cells and BxPC-3 cell-derived gemcitabine-resistant Bx-G400 cells that were treated with DMSO (diluent of the inhibitor) and GSK3β inhibitor, AR-A014418; (B) Laparotomy findings of the mice with intrapancreatic transplantation of BxPC-3 and Bx-G400 cells, respectively. The bottom panels show the mice treated with gemcitabine and AR-A014418 in combination for 8 weeks after transplantation. DAPI: 4’,6-diamidino-2-phenylindole; DMSO: dimethyl sulfoxide; GSK3β: glycogen synthase kinase 3β.