fig3

Figure 3. Inhibition or knockdown of HIF-1 suppressed the activity of PXR in hypoxia of LNCaP cells. (A) Expression of HIF-1 in LNCaP cells treatment with chrysin at the indicated concentrations and cultured under hypoxic conditions for 24 h. HIF-1 was detected by Western blot; (B) PXR activity of LNCaP cells treatment with chrysin at the indicated concentrations and cultured under hypoxic conditions for 24 h. PXR activity was determined by luciferase assay; (C) Expression of HIF-1 in LNCaP cells with stable expression shRNA against HIF-1 under hypoxic conditions. HIF-1 was detected by Western blot; (D) PXR activity of LNCaP cell lines with stable expression shRNA against HIF-1 under hypoxic conditions. The fold change was hypoxia/normoxia. The results shown are from three independent experiments. Error bars represent standard deviation. *P < 0.05. HIF-1: hypoxia-inducible factor-1; PXR: pregnane X receptor.