fig2

Figure 2. PXR’s role in hypoxia-induced chemoresistance. (A) Hypoxia increased resistance of LNCaP cells to Taxol at different dosages; (B) Effects of ketoconazole (10 M) on the sensitivity of LNCaP cells to Taxol (1 µM) under normoxic or hypoxic conditions; (C) Sensitivity of LNCaP cells with PXR knockdown to Taxol (1 M); (D) Hypoxia reduced apoptosis induced by Taxol; (E) Sensitization of hypoxic LNCaP cells toward Taxol-induced apoptosis. LNCaP cells with PXR knockdown treated with Taxol 1 µM were incubated in hypoxic condition for 48 h, and the percentage of apoptosis was determined by PI and annexin V staining followed by flow cytometry assay. The results shown were from at least three independent experiments. Error bars represent standard deviation. *P < 0.05. **P < 0.01. ns, not significant. PI: propidium iodide; PXR: pregnane X receptor.