fig4

Figure 4. Butein-instigated miR-186-5p-mediated inhibition of TWIST1 attenuated invasion of NCI-H2373 cells. (A) Representative bright-field images of Transwell invasion assay inserts 48 h after seeding of the NCI-H2373 cells. Cells were stained with crystal violet. (B) Histograms showing quantitation of the migrated NCI-H2373 cells treated as in (A) and counted at 24, 48, and 72 h after treatment started. The bound crystal violet was eluted and the absorbance at 590 nm was measured using a plate reader. The average of three experiments is shown. The percentage is relative to the relative control group except where otherwise indicated. Asterisk indicate statistical significance as follows: *P < 0.05; **P < 0.01; ns: not significant (P > 0.05).