fig1

Figure 1. Butein treatment modulated microRNA expression levels. (A) PCA plot of the microRNAs expressed in: HP1 (a); NCI-H2373 (b); and MSTO-211H (c). Blue indicates samples treated with ctrl (DMSO 0.01%), while yellow indicates samples treated with butein for 8 h at 10 µM. (B) Volcano Plot showing the microRNAs commonly modulated in all three MPM cell lines and whose perturbation by butein reached statistical significance. (C) Heatmap showing the normalized levels of the microRNAs common to the three MPM cell lines, treated as in (A). Log2 fold changes are reported. The average of the two experiments is shown. (D) PCA plot showing the distribution of the three MPM cell lines treated as in (A), after selecting the commonly modulated miRNAs.