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Figure 4. The combination of SMI and cisplatin induced Mfn2-dependent mitochondrial dynamic changes in A549/DDP cells. A549/DDP cells were pre-treated with various concentrations (13, 20, and 30 mg/mL) of SMI for 2 h and then exposed to cisplatin (25 μM) for another 24 h. (A) A549/DDP cells were labeled with the MitoTracker Green probe. The cells in the SMI and cisplatin treatment groups displayed mitochondrial spheres or ovals of widely different size, some several times larger than in the untreated cells’ mitochondrial tubules, as shown by a confocal laser scanning microscope. (B) Decrease in mitochondrial mass as determined by statistical analysis of the MitoTracker Green fluorescence intensity in (A) by ImageJ. (C) Western blotting detection and the quantified analysis by densitometry. Mean ± standard deviation, n = 3. (D) Decrease of ΔΨm in A549/DDP cells treated with a combination of SMI and cisplatin. Cells were dyed via Rhodamine 123, and the peak of fluorescence intensity of the SMI and cisplatin co-treatment groups shifted left as determined by flow cytometry and fluorescence microscopy (400×). SMI: Shenmai injection; ΔΨm: mitochondrial potential.