fig5
From: Adjuvant role of a T-type calcium channel blocker, TTA-A2, in lung cancer treatment with paclitaxel
Figure 5. TTA-A2, in combination with PTX, induced increased apoptosis in both monolayer (A, C) and spheroid cultures (B, D) of A549 cells. Cells stained with annexin showed green fluorescence and cells stained with PT showed red fluorescence. In spheroids, several cells showed co-staining with annexin and PI. I: negative control; II: PTX 10 nM; III: TTA-A2 50 nM; IV: TTA-A2 100 nM; V: PTX 10 nM + TTA-A2 100 nM. Scale: 100 μm (non-significant P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001). PTX: Paclitaxel.
