fig6

Figure 6. Showing (A) a schematic diagram of the unilamellar nanoliposome with the peptide dissolved within a stabilizing pH controlled buffer within the aqueous liposome core. Cholesterol embeds within the lipid bilayer; and a sparse PEG brush and immunogenic KDO2 lipid head group are arranged around the outer shell of the nanoliposome. The liposome is stored as a suspension in 1X PBS. It should be noted that additional lipid components are added to create either a positive or negative charge in some of the formulations. A fluorophore may also be added, which depending on the fluorophore used, may embed within the bilayer like cholesterol or be attached to a lipid and incorporate within the bilayer like PEG and KDO2. (B) shows the structure of KDO2-lipid A, which has 6 fatty acid chains and a head group on the surface.