fig1

Figure 1. Acquired nutlin-3 resistance. A549 cells were exposed to increasing concentrations of nutlin-3 for a period of 18 weeks. From these nutlin-3 exposed A549 cells, four monoclonal subclones were cultured (A); dose response curve for nutlin-3 of A549 cells, the parental resistant A549.R cells and four monoclonal subclones using the SRB assay (A549.R1-R4) (B); corresponding nutlin-3 IC₅₀ values (C); percentage survival (D); and percentage cell death of A549 and A549.R2 cells treated with idasanutlin (0-50 µmol/L) for 96 h determined by the IncuCyte ZOOM cytotoxicity assay (E). Data presented as mean +/- SD of at least 3 biological replicates