fig4

Figure 4. Plasma stability of PLAD-MLP. Gel chromatography elution profile of PLAD-MLP incubated at 37 °C for 2 h in 80% plasma. Liposomes elute in fractions 4-7; plasma proteins (green) elute mostly in fractions 8-11; free drugs elute in fractions 12-14. A: HPLC determination of MLP (red for plasma, dark blue for buffer) indicates that it remains nearly 100% intact and liposome associated during exposure to 80% human plasma for 2 h at 37 °C; B: fluorometric determination of Dox (red for plasma, dark blue for buffer) indicates that Dox remains nearly 100% liposome associated during exposure to 80% human plasma for 2 h at 37 °C. FFP: fresh frozen human plasma