fig3

Figure 3. Kinetics of MLP cleavage and MMC release by DTT. PL-MLP and PLAD-MLP (0.2 mg/mL) were incubated with DTT at 37 °C for 1 h. Samples were then processed and run on HPLC. Note the slower cleavage of MLP and lower rise of MMC for PLAD-MLP, particularly when at DTT concentrations under 0.5 mmol/L. The inset table shows a strong effect of DTT on release of Dox from PLAD-MLP, while, in the case of PLD, DTT-induced Dox release is minimal, suggesting that MLP cleavage increases the liposome permeability and causes Dox leakage