fig2

Figure 2. Analysis of the kinetics of disulfide reduction in the DUPA-FRET conjugate following internationalization by LNCaP cells. (A) Cells were either left unlabeled or (B-F) labeled with DUPA-FRET (100 nmol/L) for the indicated times at 37 °C before evaluation by confocal microscopy using 488 nm excitation. A decrease in red fluorescence accompanied by an increase in green fluorescence demonstrates release of the rhodamine from the DUPA-FRET conjugate; (B) image of cells at the 1-h time point showing a punctate distribution of fluorescence throughout the cell interior; i.e., suggesting accumulation of the conjugate within intracellular compartments